if you're playing easy/story mode, then yes. if not, you can only play response effects from your heroes or 0-cost events
in story mode, every hero gets their bonus resource before setup. in all other modes, setup is completed with no heroes having any resources.
however, you can use Eleanor's ability to cancel a treachery's when revealed effect, for example.
in the case of strider's path, it costs 1 resource, so you will only have the resources to pay for it in easy/story mode.
(1.19) Card Effects during Setup“When Revealed” effects are resolved if the cards are revealed during setup. A player can trigger responses during setup, following the normal game rules. Players cannot take Actions during setup. “When Revealed” effects that last “until the end of the phase” will last until the end of the first resource phase. Effects that last “until the end of the round”, will last until the end of the first round.
Q: Can a player use a response effect like the one on Eleanor (CORE 8) during setup?A: Yes. Responses can be triggered at any time they meet their specified prerequisite, including during setup.