1. Do rooms like the Safe Room or the Radiation Room require activation, or are they always on?
Always on.The activation marker shade on these tiles was a mistake formmy part, I should have removed it.
2. Is passing an option instead of taking an action? If you have 1 astronaut left can you move one space and then pass, if the option to move a different room and activate that room is also an option?
Passing is not allowed. If I have only one guy eft I can move or activate and stop at one action.
3. Does the escape pod tile count as a tile for purpose of running out of tiles to trigger the end game?
No, all room tiles including corridors, not shuttles.
4. Can you place a tile containing an alien next to escape pod with 1 astronaut and would it launch right away?
5. I assume you can't place a tile connected to the second door of an escape pod but not connected to another room or corridor.
Indeed as the station would not be whole anymore once the shuttle leaves