A Master Assassin of the British Brotherhood of Assassins, active in London during the Victorian era, and the younger twin brother of Evie Frye. He later became a member of Queen Victoria's Order of the Sacred Garter, and the grandfather of Lydia Frye.
Born in Crawley, where they were raised as Assassins from birth, Jacob and his sister traveled to London to take the city back from the Templars after their father died of natural causes.
Alongside Evie, Jacob led the Rooks, a criminal syndicate opposing the Templar-backed Blighters. While his sister would primarily pursue the Shroud of Eden, Jacob assassinated various prominent Templars to destroy Grand Master Crawford Starrick's influence within the city.